C6 PGD and translocations

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PGD for reciprocal and Robertsonian translocations using array comparative genomic hybridization.

BACKGROUND Fluorescence in situ hybridization (FISH) is the most widely used method for detecting unbalanced chromosome rearrangements in preimplantation embryos but it is known to have several technical limitations. We describe the clinical application of a molecular-based assay, array comparative genomic hybridization (array-CGH), to simultaneously screen for unbalanced translocation derivati...

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PGD in female carriers of balanced Robertsonian and reciprocal translocations by first polar body analysis.

Preimplantation genetic diagnosis (PGD) using the first polar body (1PB) is a modality of PGD that can be used when the woman is the carrier of a genetic disease or of a balanced chromosomal reorganization. PGD using 1PB biopsy in carriers of balanced chromosome reorganizations has not become generalized. Here, we describe our experience based on the analysis of unfertilized or fresh, non-insem...

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The origin and significance of additional aneuploidy events in couples undergoing PGD for translocations by aCGH

Diagnostic application of array-CGH in PGD for reciprocal and Robertsonian translocations has revealed 55-65% embryos with additional aneuploidies with or without translocation related imbalances. The occurrence of extra abnormalities with translocations reduces the number of embryos suitable for transfer. This study followed up 83 embryos on day 5-7 of development from 23 infertile or sub-fert...

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Can Characteristics of Reciprocal Translocations Predict the Chance of Transferable Embryos in PGD Cycles?

Translocation carriers have an increased risk of miscarriage or the birth of a child with congenital anomalies. Preimplantation genetic diagnosis (PGD) is performed in translocation carriers to select for balanced embryos and, thus, increase the chance of an ongoing pregnancy. However, a common experience is that reciprocal translocation carriers produce a high percentage of unbalanced embryos,...

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ESHRE PGD consortium best practice guidelines for amplification-based PGD.

In 2005, the European Society for Human Reproduction and Embryology (ESHRE) PGD Consortium published a set of Guidelines for Best Practice PGD to give information, support and guidance to potential, existing and fledgling PGD programmes. The subsequent years have seen the introduction of a number of new technologies as well as the evolution of current techniques. Additionally, in light of recen...

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ژورنال

عنوان ژورنال: Reproductive BioMedicine Online

سال: 2010

ISSN: 1472-6483

DOI: 10.1016/s1472-6483(10)62260-2